MABF572

Anti-CD4 Antibody (human), APC, clone OKT4

clone OKT4, from mouse

• Synonym(s): T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD antigen CD4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: Allophycocyanin conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: OKT4, monoclonal
• species reactivity: human
• packaging: pkg of 5 μL (0.125 μg/test)
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2bκ
• UniProt accession no.: P01730
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD4(920)

Description

General description

The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The OKT4 antibody is widely used as a phenotypic marker for CD4 expression. It is cross-reactive with CD4 in several non-human species, including Chimpanzee, Cynomolgus and Rhesus. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Specificity

This antibody recognizes a different epitope, and thus does not block binding of Anti-human CD4, clone RPA-T4.

Application

This mouse monoclonal antibody Anti-CD4 (human), APC & clone OKT4 validated for use in western blotting, IP, ICC, Immunofluorescence and Flow Cytometry for the detection of CD4.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD4 in one million human peripheral blood lymphocytes.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable