MABF2127

Anti-Properdin Antibody, clone 3A3E1

clone 3A3E1, from mouse

• Synonym(s): Complement factor P
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3A3E1, monoclonal
• species reactivity: baboon, rabbit, canine, human
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001138724
• UniProt accession no.: P27918
• target post-translational modification: unmodified
• Gene Information: human ... CFP(5199)

Description

General description

Properdin (UniProt: P27918; also known as Complement factor P) is encoded by the CEP (also known as PFC) gene (Gene ID: 5199) in human. (ABF185: Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization.

Specificity

Clone 3A3E1 specifically detects human properdin.

Immunogen

Purified properdin from human plasma.

Application

Inhibition Analysis: A representative lot detected Properdin in Inhibition applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Properdin in human tonsil tissue sections.

ELISA Analysis: A representative lot detected Properdin in ELISA applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).

Research Category
Inflammation & Immunology

This mouse monoclonal Anti-Properdin, clone 3A3E1 Antibody, Cat. No. MABF2127 is tested for use in Western Blotting, ELISA, Immunohistochemistry (Paraffin)for the detection of Properdin. Inhibitis the functionn of properdin by inhibiting its binding to C3b.

Quality

Evaluated by Western Blotting in purified human properdin.

Western Blotting Analysis: 1 µg/mL of this antibody detected Properdin in Purified human properdin.

Target description

~51 kDa observed; 51.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable