M8IPS4510
Multiscreen® 96 well Plate, hydrophobic PVDF membrane
pore size 0.45 μm, sterile
Synonym(s):
Hydrophobic 96-Well Plate, PVDF 96-Well Plate, PVDF Membrane Plate
About This Item
Recommended Products
material
PVDF membrane (hydrophobic)
acrylic
flat bottom wells
Quality Level
description
Clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for Avidin-biotin Linkages, DNA-binding Proteins, Affects Function, ELISPOT Assays & Protein Interaction Assays.
sterility
non-sterile
sterile
product line
MultiScreen®
feature
hydrophobic
lid
manufacturer/tradename
MultiScreen®
parameter
50-250 μL sample volume (per well)
technique(s)
ELISA: suitable
ELISPOT: suitable
activity assay: suitable (protein interaction)
filtration area
0.28 cm2
plate size
96 wells
well maximum volume
300 μL
working volume
50-250 μL
matrix
Immobilon®-P
pore size
0.45 μm pore size
binding type
high binding surface
shipped in
ambient
Related Categories
General description
HTS Filter Plates (Plate catalogue numbers that start with MS)
- MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for bar code labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics.
- Millipore’s new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays.
Classic Filter Plates (Plate catalogue numbers that start with MA)
- The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.
Accessory – MultiScreenHTS Vacuum Manifold
- The MultiScreenHTS Vacuum manifold was designed specifically to work with MultiScreenHTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.
Millicell Assay Plates
- In addition to the traditional assay plate format, filter plates are also available with cell culture trays for growing and assaying suspension and adherent cell lines. Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays.
Application
Nucleic acid
Protein
Assays
Radionucleotides
Physical form
Other Notes
Legal Information
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Articles
This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
Protocols
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
Related Content
Filtration separates substances to reduce sample complexity and increase analyte purity, improving analytical results.
This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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