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usage
sufficient for 100 tests
Quality Level
packaging
pkg of 1 96-well plate(s)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
detection method
colorimetric
fluorometric
shipped in
wet ice
storage temp.
−20°C
General description
Glucose (C6H12O6; FW: 180.16) is a very important fuel source for generating universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.
The Glucose Assay kit provides direct measurement of glucose in various biological samples (e.g. serum, plasma, body fluids). Glucose enzyme mix oxidizes glucose to generate a product which reacts with a dye to produce color and fluorescence. The generated color or fluorescence is proportional to the amount of glucose in the sample.
Components
Glucose Assay Buffer, Glucose Probe, Glucose Enzyme Mix, and Glucose Standard, and a user protocol.
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Principle
The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.
Preparation Note
• Glucose Enzyme Mix: Dissolve the Glucose Enzyme Mix in 220 µl Glucose Assay Buffer. Aliquot and store at -20°C. Use within two months.
• Glucose Probe: Supplied ready to use. Warm to room temperature to thaw the DMSO.
• Standard Curve: For colorimetric assay, dilute the Glucose Standard to 1 nmol/ml by adding 10 µl of the Glucose Standard to 990 µl of Glucose Assay Buffer, mix well. Add 0, 2, 4, 6, 8, 10 µl into each well individually. Adjust volume to 50 µl/well with Glucose Assay Buffer to generate 0, 2, 4, 6, 8, 10 nmol/well of Glucose Standard. For fluorometric assay, dilute the Glucose Standard solution to 0.1 nmol/µl by adding 10 µl of the Glucose Standard to 990 µl of Glucose Assay Buffer, mix well. Then take 20 µl into 180 µl of Glucose Assay Buffer. Mix well. Add 0, 2, 4, 6, 8, 10 µl into each well individually. Adjust volume to 50 µl/well with Glucose Assay Buffer to generate 0, 0.2, 0.4, 0.6, 0.8, 1.0 nmol/well of the Glucose Standard.
• Glucose Probe: Supplied ready to use. Warm to room temperature to thaw the DMSO.
• Standard Curve: For colorimetric assay, dilute the Glucose Standard to 1 nmol/ml by adding 10 µl of the Glucose Standard to 990 µl of Glucose Assay Buffer, mix well. Add 0, 2, 4, 6, 8, 10 µl into each well individually. Adjust volume to 50 µl/well with Glucose Assay Buffer to generate 0, 2, 4, 6, 8, 10 nmol/well of Glucose Standard. For fluorometric assay, dilute the Glucose Standard solution to 0.1 nmol/µl by adding 10 µl of the Glucose Standard to 990 µl of Glucose Assay Buffer, mix well. Then take 20 µl into 180 µl of Glucose Assay Buffer. Mix well. Add 0, 2, 4, 6, 8, 10 µl into each well individually. Adjust volume to 50 µl/well with Glucose Assay Buffer to generate 0, 0.2, 0.4, 0.6, 0.8, 1.0 nmol/well of the Glucose Standard.
Prepare test samples in a total volume of 50 µl/well with Glucose Assay Buffer. Serum can be directly diluted in Glucose Assay Buffer; typically 0.5-2 µl/assay is sufficient (normal serum contains ~5 nmol glucose/µl). We suggest testing several doses of your sample to make sure the readings are within the standard curve range.
Storage and Stability
Upon arrival store the entire contents of the kit at (-20°C). Protect from light. Note: Allow reagents warm to room temperature and briefly centrifuge vials before opening.
Other Notes
Lee, Y.S. et al. 2007.Diabetes56, 1671.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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