The His•Bind Buffer Kit is a set of pre-tested buffers designed for use with IDA-based His•Bind Resin for convenient, rapid one-step purification of proteins by metal chelation chromatography. Solutions are included for Ni2+ charging, binding, washing and elution of up to ten 2.5 ml columns.
Components
•2 × 80 ml8X Binding Buffer
•25 ml8X Wash Buffer
•50 ml4X Elute Buffer
•50 ml4X Strip Buffer
•20 ml8X Charge Buffer
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Legal Information
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
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