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Sigma-Aldrich

Protein A, Alkaline Phosphatase Conjugate

This Protein A, Alkaline Phosphatase Conjugate is validated for use in Direct Immunoassay.

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.41

Quality Level

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

General description

Native protein A conjugated to alkaline phosphatase. Can be used instead of an alkaline phosphatase-conjugated secondary antibody in many immunological assays.
Native protein A from Staphylococcus aureus conjugated to alkaline phosphatase.

Application

Direct Immunoassay (1:5000)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.

Analysis Note

Single band by SDS-PAGE

Other Notes

p-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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