1.24546

Fluorescein-5-isothiocyanate (FITC)

for biochemistry and for fluorescence microscopy

• Synonym(s): Fluorescein-5-isothiocyanate (FITC)
• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 3326-32-7
• Molecular Weight: 389.38
• MDL number: MFCD00005063
• UNSPSC Code: 12171501
• EC Index Number: 222-042-0

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥95% spectrophotometric assay
• mp: >360 °C
• εmax: 2200-2380 at 488-494 nm in 0.1 M NaOH at 0.003 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

General description

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.
FITC is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(C): Not applicable