1.15927

Ponceau S (C.I. 27195)

for microscopy Certistain^®

• Synonym(s): Ponceau S (C.I. 27195)
• Empirical Formula (Hill Notation): C₂₂H₁₂N₄Na₄O₁₃S₄
• CAS Number: 6226-79-5
• Molecular Weight: 760.57
• MDL number: MFCD00003892
• UNSPSC Code: 41116130
• EC Index Number: 228-319-2

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥80% spectrophotometric assay
• pH: 9.0 (20 °C, 10 g/L in H₂O)
• solubility: 10 g/L
• bulk density: 660 kg/m³
• εmax: 460-600 at 517-523 nm in water at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-11-14(3-8-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,25H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23?,26-21-;;;;
• InChI key: LZYGEJPERQQCIY-STNZDNLRSA-J

Description

General description

The dry dye Ponceau S (C.I. 27195) for microscopy Certistain^®, is used for the preparation of a staining solution for staining connective tissue in histological sections of human origin.
As Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 517 - 523 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; water): 460 - 600
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(C): Not applicable