05-366
Anti-PLCγ-1 Antibody, clone B-6-4
clone B-6-4, 1 mg/mL, Upstate®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B-6-4, monoclonal
species reactivity
human, rabbit, rat, bovine, mouse
manufacturer/tradename
Upstate®
concentration
1 mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PLCG1(5335)
Specificity
PLCγ-1; may also react with some non-PLC γ-1 SH-3 containing proteins
Immunogen
Purified soluble phospholipase C (PLC) g-1 from bovine brain
Application
Anti-PLCγ-1 Antibody, clone B-6-4 is an antibody against PLCγ-1 for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Lipid Signaling
Lipid Signaling
Quality
Routinely evaluated in immunoblot using acid precipitated protein from bovine brain, RIPA lysates from human A431 cells, and previously using mouse 3T3/A31 fibroblasts and rat L6 myoblasts lysates.
Target description
~135 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Certificates of Analysis (COA)
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