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05-363

Sigma-Aldrich

Anti-Cyclin E Antibody, clone HE12

clone HE12, Upstate®, from mouse

Synonym(s):

Anti-CCNE, Anti-pCCNE1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HE12, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_001238
NM_057182

UniProt accession no.

P24864

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CCNE1(898)

General description

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Specificity

human p52cyclin E

Immunogen

Recombinant human cyclin E

Application

Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC.

Quality

routinely evaluated in immunoblot on human A431 lysate

Target description

52 kDa

Linkage

Replaces: 04-223

Physical form

Format: Purified

Storage and Stability

2 years at -20°C or 6 months at 4°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hae-June Lee et al.
Radiation research, 170(5), 579-590 (2008-10-31)
The aim of this work was to identify specific genes involved in rat mammary tumors induced by dimethylbenz(a)anthracene (DMBA) or radiation. More TUNEL- and PCNA-positive cells were present in mammary tumors induced by radiation than in tumors induced by DMBA
Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells.
Maimets, T; Neganova, I; Armstrong, L; Lako, M
Oncogene null
S M Keenan et al.
The Journal of biological chemistry, 276(25), 22404-22409 (2001-04-17)
Activation of cyclin-dependent kinase 2 (CDK2)-cyclin E in the late G(1) phase of the cell cycle is important for transit into S phase. In Chinese hamster embryonic fibroblasts (IIC9) phosphatidylinositol 3-kinase and ERK regulate alpha-thrombin-induced G(1) transit by their effects
Nathaniel W Holton et al.
DNA repair, 66-67, 42-49 (2018-05-04)
Environmental exposures, reactive by-products of cellular metabolism, and spontaneous deamination events result in a spectrum of DNA adducts that if un-repaired threaten genomic integrity by inducing mutations, increasing instability, and contributing to the initiation and progression of cancer. Assessment of
Kelly R Smith et al.
Environmental toxicology, 33(3), 333-342 (2017-12-02)
The active ingredient in sunless tanning products (STPs) is a simple sugar, dihydroxyacetone (DHA). Several studies have demonstrated that DHA is absorbed within the viable layers of skin and not fully contained within the stratum corneum. Additionally, spray tanning and
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