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Key Documents

198285

Sigma-Aldrich

Nigrosin

certified by the Biological Stain Commission

Synonym(s):

Acid black 2, Nigrosin water soluble

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25 G
£28.50
100 G
£101.00

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25 G
£28.50
100 G
£101.00

About This Item

CAS Number:
Colour Index Number:
50420
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47

£28.50


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grade

certified by the Biological Stain Commission

Quality Level

form

solid

solubility

H2O: 0.1%
H2O: soluble

λmax

570 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Suitability

Certified for use in Dorner′s spore stain. Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.
Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

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Lina Hacker et al.
Journal of biomedical optics, 25(9) (2020-09-06)
Photoacoustic imaging (PAI) enables the detection of blood hemoglobin (HB) concentration and oxygenation (sO2) with high contrast and resolution. Despite the heavy use of photoacoustically determined total hemoglobin (THb) and oxygenation (sO2) biomarkers in PAI research, their relationship with underlying
Chris Campbell et al.
Biomedical optics express, 11(10), 5800-5813 (2020-11-06)
Hemozoin (Hz) is a crystal by-product of hemoglobin consumption by malaria parasites. There are currently no in vivo deep tissue sensing methods that can quantify Hz presence noninvasively, which would be advantageous for malaria research and treatment. In this work
Jesse Schallek et al.
Investigative ophthalmology & visual science, 52(3), 1325-1335 (2010-11-06)
To examine the extent to which neurovascular coupling contributes to stimulus-evoked intrinsic signals in the retina. The retinas of five adult cats were examined in vivo. Animals were anesthetized and paralyzed for imaging stability. The retinas were imaged through a
G E Freneau et al.
Animal reproduction science, 118(2-4), 176-181 (2009-09-30)
The objectives of this study were to compare two different methods of evaluating bull sperm morphology, bright-field (BF) microscopy of eosin-nigrosin (EN) stained dry-mount semen smears and differential interference phase contrast (DIC) microscopy of wet-mount semen 'fixed' in isotonic formal
Jie Guo et al.
Environmental science & technology, 42(2), 624-628 (2008-02-21)
Nonmetals reclaimed from waste phenolic cellulose paper printed circuit boards (PCBs) are used to replace wood flour in the production of phenolic molding compound (PMC). The results indicate that filling of nonmetals in PMC improves the charpy notched impact strength

Questions

1–8 of 8 Questions  
  1. What is the extinction coefficient for Nigrosin?

    1 answer
    1. regarding the extinction coefficient for product 198285,t due to the uncertain composition of the dye, providing the extinction coefficient is not feasible.

      Helpful?

  2. What is the purity of Product 198285, Nigrosin, Water Soluble?

    1 answer
    1. No dye purity is determined because the dye is a complex mixture of dyes with an unknown constitution.

      Helpful?

  3. Is Product 198285, Nigrosin, soluble in ethanol?

    1 answer
    1. Solubility is stated to be only 0.0 to 0.4% in ethanol.

      Helpful?

  4. What is the molecular weight for Product 198285, Nigrosin?

    1 answer
    1. No formula weight or empirical formula is available. The dye is innately a mixture of dyes.

      Helpful?

  5. Has Product 198285, Nigrosin, been used to test for sperm viability?

    1 answer
    1. Yes. 10% Nigrosin Water Soluble is often used with 5% aqueous Eosin Y to determine live versus dead sperm. A procedure is given in Kjeldsberg, CR and Knight JA; Body Fluids, Second Edition (ASCP Press, Chicago 1986), p 168.

      Helpful?

  6. During the certification testing for Product 198285, Nigrosin, what organism is used for testing?

    1 answer
    1. The exact organism can vary, but typically a 2-4 day old culture of a rapid spore former such as Bacillus cereus is utilized.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the solubility of Product 198285, Nigrosin, in water?

    1 answer
    1. The maximum solubility in water can vary from 5 to 10%, depending upon the lot number.

      Helpful?

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