SAB4600397

Anti-Mouse IgG (H+L), F(ab′)2 fragment, CF^™640R antibody produced in goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 100
• conjugate: CF^™ 640R conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• concentration: ~2 mg/mL
• technique(s): flow cytometry: 1-10 μg/mL; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 1-10 μg/mL; western blot: suitable
• fluorescence: λ_ex 642 nm; λ_em 662 nm
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

This product is prepared by labeling high quality F(ab′)2 fragment of goat anti-mouse IgG (H+L) with the far-red fluorescent dye CF640R. CF640R is a novel rhodamine-based dye spectrally very similar to Cy5 and Alexa Fluor 647. CF640R is much brighter than Cy5 and at least as bright as Alexa Fluor 647 when excited at 633 or 635 nm. However, the most important advantage of CF640R is its exceptional photostability. CF640R is far more photostable than the cyanine-based dyes Cy5 and Alexa Fluor 647. It is also more photostable than Atto 647N, a dye that has been extensively used in single molecule-based imaging. CF640R is also extremely water-soluble and pH-insensitive. These properties make CF640R a much better alternative to Cy5, Alexa Fluor 647 and other spectrally similar dyes.

Specificity

Binds with whole molecule mouse IgG and the light chains of other mouse immunoglobulins.

Immunogen

mouse IgG (H+L)

Application

Anti-Mouse IgG (H+L), F(ab′)2 fragment, CF^™ 640R antibody has been used in immunohistochemistry.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable