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PLN70

Sigma-Aldrich

GenElute™ Plasmid Miniprep Kit

greener alternative

sufficient for 70 purifications

Synonym(s):

GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

usage

sufficient for 70 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

greener alternative category

, Aligned

storage temp.

15-25°C

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General description

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

Features and Benefits

  • Purified plasmid DNA in less than 30 minutes
  • Purify up to 15 μg of plasmid DNA
  • No detectable genomic DNA or RNA contamination
  • No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

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Timothy A Scott et al.
Cell, 169(3), 442-456 (2017-04-22)
Fluoropyrimidines are the first-line treatment for colorectal cancer, but their efficacy is highly variable between patients. We queried whether gut microbes, a known source of inter-individual variability, impacted drug efficacy. Combining two tractable genetic models, the bacterium E. coli and the nematode C. elegans
Shree Vidhya Srinivasan et al.
Heliyon, 5(11), e02863-e02863 (2019-12-18)
Pregnancy-associated glycoproteins (PAGs) secreted from conceptus specific trophoblast cells are widely accepted biomarkers of ruminants. Limited information of PAGs in buffalo warrants further investigation for the development of sensitive homologous early pregnancy-specific diagnostic immunoassay. This experiment was thus designed to
Cloning and Characterization of Serine-rich Entamoeba histolytica Protein Gene from an Iranian E. histolytica Isolate
Sima Rasti
Pakistan journal of biological sciences: PJBS, 9, 654-658 (2006)
Genetic determinants of resistance to fusidic acid among clinical bacteremia isolates of Staphylococcus aureus.
Lannergard J
Antimicrobial Agents and Chemotherapy, 53(5), 2059-2065 (2009)
Comparison of three molecular methods for the detection of pilchard herpesvirus in archived paraffin-embedded tissue and frozen tissue.
Crockford M
Diseases of Aquatic Organisms, 82(1), 37-44 (2008)
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Protocols

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

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