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P1526

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

peroxidase substrate, chromogenic, solid

Synonym(s):

OPD

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
Beilstein:
3912045
EC Number:
210-418-7
MDL number:
MFCD00012966
UNSPSC Code:
12352204
PubChem Substance ID:
24898213
NACRES:
NA.83

product name

o-Phenylenediamine dihydrochloride, peroxidase substrate

Quality Level

300

form

solid

color

white to off-white

mp

258 °C (dec.) (lit.)

storage temp.

−20°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in enzyme-linked immunosorbent assay (ELISA) procedures.

Caution

May darken in storage.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Areano Ethério Moreira de Farias et al.
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Goat breeding in the Northeast region of Brazil plays an important socioeconomic role. However, there are significant losses caused by sanitary deficits and infectious diseases, particularly caseous lymphadenitis (CL). Although CL is considered endemic in Northeastern Brazil, a comprehensive and
Bikash R Sahu et al.
The Journal of infectious diseases, 198(3), 434-443 (2008-07-01)
Filaria-specific antibodies of immunoglobulin (Ig) G, IgE, and IgM isotypes have been correlated with acquired immunity in the literature, but the status of filaria-specific IgA and its role in human filariasis has not been addressed. The present study attempts to
Andrew K I Falconar et al.
Clinical and vaccine immunology : CVI, 13(9), 1044-1051 (2006-09-09)
We compared dengue virus (DV) isolation rates and tested whether acute primary (P) and acute/probable acute secondary (S/PS) DV infections could be correctly classified serologically when the patients' first serum (S1) samples were obtained 1 to 3 days after the
E O'Connor et al.
Journal of immunological methods, 229(1-2), 155-160 (1999-11-11)
The ability to detect a protein is always limited to the sensitivity of the assays available. Progress in improving the sensitivity of protein detection will allow a more complete understanding of biological systems. Of particular interest to the field of
Guy Mollett et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 69(7), 1130-1135 (2018-12-13)
Visceral leishmaniasis (VL), caused by the Leishmania donovani complex, is a fatal, neglected tropical disease that is targeted for elimination in India, Nepal, and Bangladesh. Improved diagnostic tests are required for early case detection and for monitoring the outcomes of
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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