M9400

β-Mannosidase from Helix pomatia

5-30 units/mL, ammonium sulfate suspension, crude extract

• Synonym(s): β-D-Mannoside mannohydrolase
• CAS Number: 9025-43-8
• Enzyme Commission number: 3.2.1.25 (BRENDA, IUBMB)
• MDL number: MFCD00130638
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• form: ammonium sulfate suspension
• Quality Level: 200
• quality: crude extract
• mol wt: 94 kDa
• concentration: 5-30 units/mL
• foreign activity: β-N-acetylglucosaminidase and α-mannosidase <1.0%
• storage temp.: 2-8°C

Description

General description

β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.

Application

β-Mannosidase from Helix pomatia has been used:

• in the digestion of glycans for enzymatic analysis
• in the treatment of labeled monogalactosyl Manno-oligosaccharides
• in non-mammalian glycosidase inhibition assays

Biochem/physiol Actions

β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ containing 10 mM sodium acetate, pH approx. 4.0

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves