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FLAAB

Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer

lyophilized powder

Synonym(s):

ATP Assay Dilution Buffer, Assay Mix Buffer, Assay Mix for ATP

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.84

form

lyophilized powder

Quality Level

technique(s)

activity assay: suitable

storage temp.

−20°C

General description

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer is a component of the ATP bioluminescent assay kit that may be employed for the quantitative bioluminescent determination of ATP in experimental samples.

Application

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer has been used to determine the level of adenosine kinase activity in isolated sections of mouse hippocampi. It has also been used to measure the ATP content in oocytes and harderian gland(HG) tissue homogenates.

Components

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer contains MgSO4, dithiothreitol (DTT), ethylene diamine tetraacetic acid (EDTA, bovine serum albumin (BSA), and tricine buffer salts.

Reconstitution

Reconstitute with 50 mL of sterile water.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Andrea Boyd-Tressler et al.
The Journal of biological chemistry, 289(39), 27246-27263 (2014-08-13)
Anti-tumor immune responses have been linked to the regulated release of ATP from apoptotic cancer cells to engage P2 purinergic receptor signaling cascades in nearby leukocytes. We used the Jurkat T cell acute lymphocytic leukemia model to characterize the role
Peter Stief et al.
BMC microbiology, 14, 35-35 (2014-02-13)
A wealth of microbial eukaryotes is adapted to life in oxygen-deficient marine environments. Evidence is accumulating that some of these eukaryotes survive anoxia by employing dissimilatory nitrate reduction, a strategy that otherwise is widespread in prokaryotes. Here, we report on
Nicolette Gouder et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24(3), 692-701 (2004-01-23)
Endogenous adenosine in the brain is thought to prevent the development and spread of seizures via a tonic anticonvulsant effect. Brain levels of adenosine are primarily regulated by the activity of adenosine kinase. To establish a link between adenosine kinase
Hana M Russo et al.
Journal of immunology (Baltimore, Md. : 1950), 197(4), 1353-1367 (2016-07-08)
Canonical inflammasome activation induces a caspase-1/gasdermin D (Gsdmd)-dependent lytic cell death called pyroptosis that promotes antimicrobial host defense but may contribute to sepsis. The nature of the caspase-1-dependent change in plasma membrane (PM) permeability during pyroptotic progression remains incompletely defined.
Nobuhiro Moro et al.
Experimental and therapeutic medicine, 21(6), 575-575 (2021-04-15)
The aim of the current study was to determine effects of mild traumatic brain injury (TBI), with or without blockade of purinergic ATP Y1 (P2Y1) receptors or store-operated calcium channels, on extracellular levels of ATP, glutamate, glucose and lactate. Concentrations

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