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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-macrophage, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Not specified
Cell Line Description
The antibody recognises a cytoplasmic antigen on monocytes and on most rat macrophage sub-populations. Please note that this hybridoma produces antibody in very low amounts (0.1 μg/ml).
Immunogen
Highly purified recombinant human tumour necrosis factor alpha
Application
Not specified
Culture Medium
RPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS) (heat inactivated)
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C.
Other Notes
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