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Key Documents

B3148

Sigma-Aldrich

Anti-Goat/Sheep IgG−Biotin antibody, Mouse monoclonal

clone GT-34, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Biotin antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GT-34, monoclonal

form

buffered aqueous solution

species reactivity

sheep, goat, bovine

should not react with

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

technique(s)

direct ELISA: 1:5,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.
The product binds to all goat/sheep IgG1

Application

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse has been used in:
  • enzyme linked immuno sorbent assay (ELISA)
  • immunoblotting
  • dot blotting
  • immunohistochemistry
  • immunocytochemistry

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse may be used for ELISA at a minimum working dilution of 1:5000. It was used at a working dilution of 1:1000 to visualize collagen type IV in mouse digit sections.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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