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A8914

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)−Agarose antibody produced in goat

affinity isolated antibody, saline suspension

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About This Item

MDL number:
MFCD00162781
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

200

conjugate

agarose conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

saline suspension

extent of labeling

~1 mg per mL

technique(s)

immunoelectrophoresis: suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

capacity

≥0.25 mg/mL, resin binding capacity (rabbit IgG)

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgG antibodies are found in all body fluids and are capable of fighting bacterial and viral infection. It is the only type of antibody capable of crossing the placenta and immunising the foetus. Anti-rabbit IgG (whole molecule)-agarose antibody can be used in immunoprecipitation. IgGs are added to nuclear extracts of breast cancer cell lines then immunoprecipitation carried out. Anti-rabbit IgG (whole molecule)-agarose antibody can react specifically to all rabbit immunoglobulin.

Immunogen

Purified rabbit IgG.

Application

Anti-rabbit IgG (whole molecule)-agarose antibody can be used in immunoprecipitation and immunoelectrophoresis.
Protein depletion from mouse intestinal cytosol samples was accomplished by incubating the samples with a specific primary and the pulling down with agarose-linked goat anti-rabbit IgG.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shahzad Siddiqi et al.
Journal of lipid research, 51(5), 1093-1100 (2009-12-08)
The rate-limiting step in the transit of dietary fat across the intestinal absorptive cell is its exit from the endoplasmic reticulum (ER) in a specialized ER-to-Golgi transport vesicle, the prechylomicron transport vesicle (PCTV). PCTV bud off from the ER membranes
The TACC proteins are coregulators of the Retinoid X Receptor b.
Ananthalaksmy K Vettaikkorumakankauv
Cancer Therapy, 6, 805-816 (2008)
Ronja Markworth et al.
Journal of cell science, 134(20) (2021-09-07)
Axonal survival and growth requires signalling from tropomyosin receptor kinases (Trks). To transmit their signals, receptor-ligand complexes are endocytosed and undergo retrograde trafficking to the soma, where downstream signalling occurs. Vesicles transporting neurotrophic receptors to the soma are reported to
Ronja Markworth et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(30), 5842-5860 (2019-05-28)
Neural circuit development involves the coordinated growth and guidance of axons. During this process, axons encounter many different cues, but how these cues are integrated and translated into growth is poorly understood. In this study, we report that receptor signaling

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