The activity of this product varies from lot to lot with the minimum amount in a 1000 unit vial being 3.33 mg. This is based on the maximum allowable activity of 300 units per milligram protein and the maximum 100% protein. The maximum amount will be 16 mg per 1000 unit vial. This is based on the minimum allowable activity of 100 units per milligram protein and the minimum allowable protein content of 60%. Please see the link below to access a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/a3809#product-documentation
A3809
Asparaginase from Escherichia coli
lyophilized powder, 100-300 units/mg protein (biuret)
Synonym(s):
L-Asparagine Amidohydrolase
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About This Item
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form
lyophilized powder
Quality Level
specific activity
100-300 units/mg protein (biuret)
composition
Protein, ≥60%
storage temp.
2-8°C
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Application
Biochem/physiol Actions
Asparaginase (ASNase) products are usually obtained from Escherichia coli and Erwinia chrysanthemi. These enzymes can block the synthesis of protein in tumor cells. It shows high activity in the G1 phase of the cell cycle.[6] It is capable of causing pancreatitis in leukemia patients.[2]
Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia [7]. Product A3809 is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride.
Unit Definition
One unit will liberate 1.0 μmole of ammonia from L-asparagine per min at pH 8.6 at 37 °C.
Physical form
Lyophilized powder containing sodium chloride
Preparation Note
Chromatographically purified
Analysis Note
Protein determined by biuret.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 2 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Metabolic reprogramming plays an essential role on lymphoma progression. Dysregulation of glutamine metabolism is implicated in natural-killer T-cell lymphoma (NKTCL) and tumor cell response to asparaginase-based anti-metabolic treatment. To understand the metabolomic alterations and determine the potential therapeutic target of
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Asparaginase is fundamental to the treatment of haematological malignancies. However, little has been studied on the effects that asparaginase could exert on solid tumours. Thus, this study aimed to evaluate the effects of asparaginase on an oral carcinoma cell line.
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What is the actual quantity (in the gram) in 1 unite of the enzyme. Because, we want use the enzyme in gram. such as i have 300 unit of this enzyme. So please tell what is the exact quantity in gram
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I would like to obtain the asparaginase (A3809-1KU) enzymatic assay protocol.
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Please see the protocol at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/369/983/a3809enz.pdfHelpful?
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What is the molecular weight of this enzyme (Product No. A3809)?
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This enzyme consists of a tetramer composed of four identical subunits of 34,080 Daltons each. The molecular weight of the tetramer is 136,320 Daltons.
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What is the source of this enzyme (Product No. A3809)?
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This enzyme is obtained from a natural extraction of E. coli. It is not from a recombinant source.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How should this enzyme (Product No. A3809) be reconstituted?
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Prior to determining the activity of this enzyme, Sigma reconstitutes it directly in deionized water. Solutions should be prepared fresh, as the stability of stock solutions has not been evaluated.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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