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P1254

Sigma-Aldrich

Brij® L23

suitable for Stein-Moore chromatography

Synonym(s):

Brij 35, C12E23, Polyoxyethylene (23) lauryl ether

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About This Item

CAS Number:
9002-92-0
MDL number:
MFCD00080891
UNSPSC Code:
12161900
PubChem Substance ID:
24898176
NACRES:
NA.21
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description

non-ionic

Quality Level

200

form

solid

mol wt

micellar avg mol wt 48,000
estimated mol wt 1198

aggregation number

20-40

CMC

91 μM

transition temp

cloud point >100 °C

HLB

16.9

suitability

suitable for Stein-Moore chromatography

SMILES string

CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO

InChI

1S/C58H118O24/c1-2-3-4-5-6-7-8-9-10-11-13-60-15-17-62-19-21-64-23-25-66-27-29-68-31-33-70-35-37-72-39-41-74-43-45-76-47-49-78-51-53-80-55-57-82-58-56-81-54-52-79-50-48-77-46-44-75-42-40-73-38-36-71-34-32-69-30-28-67-26-24-65-22-20-63-18-16-61-14-12-59/h59H,2-58H2,1H3

InChI key

IEQAICDLOKRSRL-UHFFFAOYSA-N

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Application

Non-ionic detergent. Useful for the extraction of membrane proteins. Typically used in the range of 0.1-1%.

Legal Information

Brij is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

>300.2 °F - open cup

Flash Point(C)

> 149 °C - open cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000299534
0000299534
0000257340
0000000270
0000241189

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K Yamamoto et al.
Biochimica et biophysica acta, 790(3), 208-218 (1984-11-09)
Two forms of an acid proteinase have been purified from human erythrocyte membranes by a simple method involving selective extraction with 0.5% Brij 35, affinity chromatography on pepstatin A-Sepharose 4B, and chromatography on Sephacryl S-200 and DEAE-Sephadex G-100. One species
T Tobimatsu et al.
Archives of biochemistry and biophysics, 347(1), 132-140 (1997-11-05)
Recombinant adenosylcobalamin-dependent diol dehydratase of Klebsiella oxytoca overexpressed in Escherichia coli was purified to homogeneity. The enzyme has a low solubility and was extracted from the crude membrane fraction with 1% Brij 35 in a high recovery. Subsequent chromatography on
Jacomina W van den Esschert et al.
Annals of surgery, 255(2), 311-318 (2012-01-14)
To compare the safety and hypertrophy response after portal vein embolization (PVE) using 2 absorbable and 3 permanent embolization materials. Portal vein embolization is used to increase future remnant liver volume preoperatively. Application of temporary, absorbable embolization materials could be
Jun Koizumi et al.
AJR. American journal of roentgenology, 199(1), 200-207 (2012-06-27)
Balloon-occluded retrograde transvenous obliteration has been traditionally based on liquid sclerotherapy. However, overdose and systemic spillage of liquid sclerosant can cause severe complications, such as hemolysis, which lead to hemoglobinuria, allergy, acute respiratory distress syndrome, and other disorders. The purpose
Otte Brosjö et al.
Acta orthopaedica, 84(5), 502-505 (2013-11-01)
Recent data suggest that percutaneous sclerotherapy is a safe alternative to surgery for treatment of aneurysmal bone cysts (ABCs). We present our experience of this method. We retrospectively analyzed data from 38 consecutive patients treated with repeated injections of polidocanol.
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. How to prepare a 1% solution of Brij 35?

    1 answer

    1. To prepare a 1% solution of Brij 35, first verify the concentration of the Brij 35 solution. Once the concentration is known, can use water as the diluent to prepare the 1% solution of Brij 35. If the Brij 35 solution is not already at the desired 1% concentration, dilute it accordingly with water.

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