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MAB1293

Sigma-Aldrich

Anti-DNA Antibody, double stranded, clone AE-2

clone AE-2, Chemicon®, from mouse

Synonym(s):

Double-stranded DNA antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AE-2, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG3

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Double stranded DNA. MAB1293 produces a homogeneous staining pattern in the nucleus of normal and malignant cells.

Immunogen

Epitope: double stranded
Nuclei of Raji Burkitt′s lymphoma cells

Application

Anti-DNA Antibody, double stranded, clone AE-2 is a high quality Mouse Monoclonal Antibody for the detection of DNA & has been validated in ICC & IHC.
Immunocytochemistry: paraformaldehyde fixed cell preparations

Immunohistochemistry: paraformaldehyde fixed frozen tissue sections

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected double stranded DNA in human prostrate and tonsil tissue sections.

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25 NaCl and 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Analysis Note

Control
POSITIVE CONTROL: Human tonsil

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lateral elements inside synaptonemal complex-like polycomplexes in ndt80 mutants of yeast bind DNA.
Bhuiyan, H; Dahlfors, G; Schmekel, K
Genetics null
Manuel Adrian Suter et al.
Scientific reports, 11(1), 7243-7243 (2021-04-02)
Deficiencies in DNA repair and DNA degrading nucleases lead to accumulation of cytosolic DNA. cGAS is a critical DNA sensor for the detection of cytosolic DNA and subsequent activation of the STING signaling pathway. Here, we show that the cGAS-STING
Yang Liu et al.
Scientific reports, 6, 18696-18696 (2016-01-05)
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Hazel Quek et al.
Journal of leukocyte biology, 101(4), 927-947 (2016-11-30)
Mutations in the ataxia-telangiectasia (A-T)-mutated (ATM) gene give rise to the human genetic disorder A-T, characterized by immunodeficiency, cancer predisposition, and neurodegeneration. Whereas a series of animal models recapitulate much of the A-T phenotype, they fail to present with ataxia
Si-Yu Wu et al.
Journal for immunotherapy of cancer, 9(7) (2021-07-30)
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer and lacks definite treatment targets. Tumor immune microenvironment (TIME) heterogeneity has a profound impact on the immunotherapy response. Tumors with non-inflamed TIME derive limited benefit from immunotherapy. However

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