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CBL114F

Sigma-Aldrich

Mouse Anti-Human IgA Antibody, clone M24A, heavy chain, FITC conjugated

clone M24A, Chemicon®, from mouse

Synonym(s):

Anti-human IgA FITC

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

M24A, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Specificity

This antibody is specific for the heavy chain of human immunoglobulin A, and shows no cross-reactivity with intact human IgM or IgG, kappa or lambda light chains

Application

Immunohistological staining of IgA expressing cells in tissue sections.

Identification of surface IgA on B lymphocytes by direct Immunofluorescence.

ELISA

Flow Cytometry

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
This Mouse anti-Human IgA Antibody, clone M24A, heavy chain, FITC conjugated is validated for use in ELISA, FC, IF, IH for the detection of Human IgA.

Physical form

The conjugate is supplied in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.

Storage and Stability

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Philip A Mudd et al.
Cell, 185(4), 603-613 (2022-01-14)
SARS-CoV-2 mRNA vaccines induce robust anti-spike (S) antibody and CD4+ T cell responses. It is not yet clear whether vaccine-induced follicular helper CD4+ T (TFH) cell responses contribute to this outstanding immunogenicity. Using fine-needle aspiration of draining axillary lymph nodes from
Jackson S Turner et al.
Nature, 595(7867), 421-425 (2021-05-25)
Long-lived bone marrow plasma cells (BMPCs) are a persistent and essential source of protective antibodies1-7. Individuals who have recovered from COVID-19 have a substantially lower risk of reinfection with SARS-CoV-28-10. Nonetheless, it has been reported that levels of anti-SARS-CoV-2 serum
Jackson S Turner et al.
Nature, 596(7870), 109-113 (2021-06-29)
SARS-CoV-2 mRNA-based vaccines are about 95% effective in preventing COVID-191-5. The dynamics of antibody-secreting plasmablasts and germinal centre B cells induced by these vaccines in humans remain unclear. Here we examined antigen-specific B cell responses in peripheral blood (n = 41) and draining lymph
SARS-CoV-2 Omicron boosting induces de novo B cell response in humans.
Alsoussi, et al.
Nature, 617, 592-598 (2023)
Joana P Bernardes et al.
Immunity, 53(6), 1296-1314 (2020-12-10)
Temporal resolution of cellular features associated with a severe COVID-19 disease trajectory is needed for understanding skewed immune responses and defining predictors of outcome. Here, we performed a longitudinal multi-omics study using a two-center cohort of 14 patients. We analyzed

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