HPLC Analysis of Folinic Acid Enantiomers on Astec^® CHIROBIOTIC^® T (RP mode)

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] 1% triethylamine acetate, pH 4.5; [B] ethanol; (50:50, A:B)

flow rate

1 mL/min

column temp.

20 °C

detector

UV, 254 nm

injection

5 μL

sample

1.25 mg/mL in methanol