HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase
Matériaux
Colonne analytique
Réf. du produit
Description
Tarif
Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column
2.7 μm particle size, L × I.D. 5 cm × 4.6 mmÉtalon
Réf. du produit
Description
Tarif
Oxazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Alprazolam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Clonazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Diazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)
mobile phase
[A] water; [B] acetonitrile; (62:38, A:B)
flow rate
1.4 mL/min
pressure
2600 psi (179 bar)
column temp.
35 °C
detector
UV, 250 nm
injection
2 μL
sample
0.1 g/mL of each in 20% methanol
Description
Remarque sur l'analyse
The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.
Informations légales
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany