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Solid-phase hybridization efficiency improvement on the magnetic nanoparticle surface by using dextran as molecular arms.

Journal of biomedical nanotechnology (2013-09-26)
Zhiyang Li, Haowen Yang, Nongyue He, Wenbiao Liang, Chao Ma, Muhammad Ali A Shah, Yongjun Tang, Song Li, Hongna Liu, Hesheng Jiang, Yafen Guo
PMID24059094
RÉSUMÉ

For reducing the steric hindrance and nonspecific binding of the target DNA, the dextran was used as molecular arms to be immobilized on the surface of magnetic nanoparticles (MNPs). Magnetic separation was used in preparation of dextran-MNPs (DMNPs). Aspartic acid and aminated DNA probe were successively modified on the dextran immobilized on the surface of MNPs. These probe-DMNPs were successfully applied to detect biotin-labeled PCR product of E. coli O157:H7 genome by hybridization. Then the complexes were bonded with streptavidin-modified alkaline phosphatase (ALP-SA). Finally the chemiluminescent signals were detected by adding 3-(2-spiroadamantane)-4-methoxy-4- (3-phosphoryloxy) phenyl-1,2-dioxetane (AMPPD). The results showed that this method had a good specificity, and higher sensitivity than that when only MNPs were used as solid carriers.

MATÉRIAUX
Référence du produit
Marque
Description du produit
31392
Sigma-Aldrich
Dextrane from Leuconostoc spp., Mr 450,000-650,000
31390
Sigma-Aldrich
Dextrane from Leuconostoc spp., Mr ~70,000
31389
Sigma-Aldrich
Dextrane from Leuconostoc spp., Mr ~40,000
D9260
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, average mol wt 9,000-11,000
D5376
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, average mol wt 1,500,000-2,800,000
D8821
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, average mol wt 60,000-76,000
D1662
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, average mol wt 35,000-45,000
31388
Sigma-Aldrich
Dextrane from Leuconostoc spp., Mr ~6,000
D4876
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, average mol wt 150,000
00271
Supelco
Dextran, analytical standard, for GPC, 25,000
09184
Sigma-Aldrich
Dextrane from Leuconostoc spp., Mr ~100,000
00269
Supelco
Dextran, analytical standard, for GPC, 5,000
31398
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, Mr ~200,000
00268
Supelco
Dextran, analytical standard, for GPC, 1,000
31387
Sigma-Aldrich
Dextrane from Leuconostoc spp., Mr 15,000-25,000
31397
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, Mr ~60,000
31425
Supelco
Dextrane from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 670,000
31394
Sigma-Aldrich
Dextran, enzymatic synth.
D8802
Sigma-Aldrich
Dextran solution from Leuconostoc mesenteroides, 20 % (w/w) (Autoclaved)
00894
Supelco
Dextran, analytical standard, for GPC, 270,000
31417
Supelco
Dextrane from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 5,000
00895
Supelco
Dextran, analytical standard, for GPC, 410,000
D3759
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, average mol wt 48,000-90,000
00893
Supelco
Dextran, analytical standard, for GPC, 150,000
00891
Supelco
Dextran, analytical standard, for GPC, 50,000
31419
Supelco
Dextrane from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 25,000
31416
Supelco
Dextrane from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 1,000
D4626
Sigma-Aldrich
Dextrane from Leuconostoc mesenteroides, average mol wt 15,000-30,000
31430
Supelco
Dextran, analytical standard, for GPC, Set Mp 1,000-400,000
00270
Supelco
Dextran, analytical standard, for GPC, 12,000