Lung Section Staining and Microscopy.

Bio-protocol (2017-11-25)

Xiaofeng Zhou, Bethany B Moore

RÉSUMÉ

Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson's trichrome staining on lung sections.

MATÉRIAUX

Référence du produit

Marque

Description du produit

Sigma-Aldrich

Acide acétique, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Acide chlorhydrique, ACS reagent, 37%

Sigma-Aldrich

Iron(III) chloride, reagent grade, 97%

Sigma-Aldrich

Acide citrique, ACS reagent, ≥99.5%

Sigma-Aldrich

Aluminum potassium sulfate dodecahydrate, ACS reagent, ≥98%

Sigma-Aldrich

Hematoxylin, certified by the Biological Stain Commission

Sigma-Aldrich

Eosin Y, Dye content ~99 %

Sigma-Aldrich

Phosphate de sodium monobasic, BioXtra, ≥99.0%