Accéder au contenu
Merck
Toutes les photos(3)

Documents

52798-U

Supelco

HybridSPE®-Phospholipid

Small Volume 96-well Plate, bed wt. 15 mg, volume 0.8 mL, pk of 20

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(3)

About This Item

Code UNSPSC :
41115712
Nomenclature NACRES :
NB.21

Forme

solid

Composition

bed wt., 15 mg

Conditionnement

pk of 20

Technique(s)

solid phase extraction (SPE): suitable

Volume

0.8 mL

Groupe de la matrice active

zirconia-based phase

Application(s)

food and beverages

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Description générale

HybridSPE-Phospholipid technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is loaded on the HybridSPE-Phospholipid 96-well plate or cartridge which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids. The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE-Phospholipid stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS-MS analysis.

The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.

Caractéristiques et avantages

  • Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
  • Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
  • 2-3 step generic procedure
  • Minimal to no method development
  • Available in 96-well and 1 mL cartridge dimensions

Informations légales

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Choose from one of the most recent versions:

Certificats d'analyse (COA)

Lot/Batch Number
14928301
11877101
8509601
7779701

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 1

1 of 1

Module d’extraction sous vide PlatePrep 96-puits Starter kit

Supelco

575650-U

Module d′extraction sous vide PlatePrep 96-puits

Identification and elimination of ion suppression in the quantitative analysis of sirolimus in human blood by LC/ESI-MS/MS
Mano, N., et al.
Journal of Chromatography. B, Biomedical Applications, 879 (13-14), 968-974 (2011)
Improved sensitivity of sedimentary phospholipid analysis resulting from a novel extract cleanup strategy
Zhu, Z., et al.
Organic Geochemistry, 65, 46-52 (2013)
Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry
Ismaiel, O., et al.
Journal of Chromatography. B, Biomedical Applications, 878 (31), 3303-3316 (2010)
Determination of carboplatin in human plasma using HybridSPE-precipitation along with liquid chromatography-tandam mass spectrometry
Hongliang, J,, et al.
Journal of Chromatography. B, Biomedical Applications, 879 (22), 2162-2170 (2011)
Pankaj K Kanaujia et al.
Journal of chromatography. A, 1218(38), 6612-6620 (2011-08-25)
Selective extraction and enrichment of nerve agent degradation products has been achieved using zirconia based commercial solid-phase extraction cartridges. Target analytes were O-alkyl alkylphosphonic acids and alkylphosphonic acids, the environmental markers of nerve agents such as sarin, soman and VX.
Afficher tous les articles scientifiques apparentés

Articles

Ion-Suppression & Phospholipid Contamination

An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

Protocoles

Enrichment of Phospholipids in Biological Samples Using HybridSPE-PL

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique