ZRB2856

Anti-Prohibitin 1 Antibody, clone 1N19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : PHB, Prohibitin
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1N19, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 29.80 kDa; observed mol wt ~32 kDa
• Produit purifié par: using Protein A
• Espèces réactives: mouse, human, rat
• Réactivité de l'espèce (prédite par homologie): chicken, bovine
• Conditionnement: antibody small pack of 25
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgGκ
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_001268425
• Numéro d'accès UniProt: P35232
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... PHB1(5245)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1N19 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects Prohibitin 1. It targets an epitope within 25 amino acids from the C-terminal region of the protein.

Immunogène

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human Prohibitin 1.

Application

Quality Control Testing

Evaluated by Western Blotting in Caco-2 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Prohibitin 1 protein in Caco-2 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Prohibitin 1 in lysates from PC-12 cells, human liver tissue, and mouse heart tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Prohibitin 1 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Prohibitin 1 in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

Prohibitin 1 (UniProt: P35232; also known as Prohibitin) is encoded by the PHB1 (also known as PHB) gene (Gene ID: 5245) in human. PHB gene encodes an antiproliferative protein that controls several cellular control activities such as cell proliferation, cristae morphogenesis and the functional integrity of mitochondria. PHB1 is predominantly localized in the inner membrane of mitochondria, and together with PHB2 forms large ring complexes (prohibitin complexes) and functions as a chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan. PHB1 has also been observed in the nucleus, cytoplasm and plasma membrane. The presence of PHB1 in the nucleus and at the cell surface suggests additional roles in nuclear signaling and cell surface receptor-mediated signaling. The expression of PHB1 is widespread, being found in a variety of tissues and cell types. It is ubiquitously expressed in both adult and embryonic tissues, suggesting its fundamental role in cellular processes. Prohibitin is an inhibitor of DNA synthesis and is a potential tumor suppressor gene. It associates with Rb family proteins and suppresses their binding to E2F transcription factors, thus inhibiting E2F-mediated transcription and cell proliferation. Overexpression of PHB1 has been observed in various cancers, where it may contribute to the regulation of apoptosis and cell proliferation, affecting tumor growth and progression. Expression levels of prohibitin in fibroblasts have been found to decrease heterogeneously as cellular aging occurs. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable