ZMS1073

Anti-Calretinin Antibody, clone 6B8.2 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 6B8.2, recombinant monoclonal
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 31.41 kDa; observed mol wt ~31 kDa
• Espèces réactives: rat, mouse, human
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: Unknown
• Numéro d'accès UniProt: P47728
• Autre catégorie plus écologique: , Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 6B8.2 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Calretinin.

Immunogène

Recombinant rat Calretinin.

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Calretinin in Mouse brain tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Calretinin in lysates from rat brain, human brain, and U2OS cells.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Calretinin in rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calretinin in E18 Rat cortical cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Calretinin in rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Calretinin, clone 6B8.2 ZooMAb^®, Cat. No. ZMS1073, is a recombinant Mouse monoclonal antibody that detects Calretinin and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Description de la cible

Calretinin (UniProt: P47728; also known as CR) is encoded by the Calb2 gene (Gene ID: 117059) in rat. Calretinin belongs to the calbindin protein family and acts as a calcium-binding protein. It is expressed in both central and peripheral nervous system and is found abundantly in auditory neurons. Overexpression of calretinin has been reported in most types of malignant mesothelioma. It is involved in regulating cytosolic calcium ion concentrations and has multiple calcium-binding domains known as EF-hands. Its expression is shown to be regulated at the level of transcription by nuclear respiratory factor 1 (NRF-1) and E2F2 transcription factors. In rat striatum calretinin neurons form a distinct subset of medium-sized, aspiny, GABAergic interneurons. Calretinin can also serve as an important differential diagnostic tool for cystic and solid ameloblastic tumors. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable