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Y1376

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without leucine

Synonyme(s) :

Synthetic Yeast Growth Media

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About This Item

Numéro MDL:
MFCD01095679
Code UNSPSC :
12000000
Nomenclature NACRES :
ND.02

Niveau de qualité

100

Forme

powder

Application(s)

food and beverages

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Catégories apparentées

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Composants

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for leucine. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).

Autres remarques

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Quantité

1.6 g will supplement 1 liter of medium.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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