X1254
Anti-XPA (C-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonyme(s) :
Anti-XP1, Anti-XPAC, Anti-Xeroderma pigmentosum, complementary group A
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
IgG fraction of antiserum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Poids mol.
antigen 35 kDa (doublet)
Espèces réactives
human
Technique(s)
western blot: 1:1,000-1:2,000 using Jurkat cell lysates
western blot: 1:250-1:500 using Rat1 cell lysates
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... XPA(7507)
mouse ... Xpa(22590)
rat ... Xpa(298074)
Description générale
Xeroderma pigmentosum group A-complementing protein (XPA) or DNA repair protein complementing XP-A cells human excision repair protein. XPA encodes a hydrophilic metalloprotein. It displays a C4 zinc finger motif, a central globular domain and has disordered regions in the N and C-terminus. The gene XPA is localized in human chromosome 9q22.33.
Spécificité
Anti-XPA (C-terminal) specifically recognizes human XPA.
Immunogène
synthetic peptide corresponding to amino acids 257-273 of human XPA, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the mouse and rat sequences by one amino acid.
Application
Anti-XPA (C-terminal) antibody produced in rabbit has been used in immunolabeling and immunoblotting.
Actions biochimiques/physiologiques
Xeroderma pigmentosum group A-complementing protein (XPA) interacts with nucleotide excision repair (NER) subunits, such as replication protein A (RPA), excision repair complementing 1 protein (ERCC1), and transcription factor II (TFIIH). However, it is widely accepted that the Xeroderma pigmentosum group C-complementing protein (XPC)-human Rad23 homolog (hHR23B) complex recognizes the DNA damage-induced helical distortion. After this, the transcription factor IIH (TFIIH), XPA (possibly in its homodimeric form), and replication protein A (RPA) arrive sequentially at the site of damage. XPA interacts directly with DNA via the zinc finger motif. RPA coordinates with XPA in the positioning in the nucleotide excision repair (NER) bubble. Defects in the excision repair leads to photosensitivity syndrome called xeroderma pigmentosum (XP).
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Chin-Ju Park et al.
The FEBS journal, 273(8), 1600-1608 (2006-04-21)
Xeroderma pigmentosum (XP) is an inherited disease in which cells from patients exhibit defects in nucleotide excision repair (NER). XP proteins A-G are crucial in the processes of DNA damage recognition and incision, and patients with XP can carry mutations
Reinhart Speeckaert et al.
Pigment cell & melanoma research, 27(4), 512-524 (2014-03-13)
Hyperpigmentation is a key feature in a variety of inherited and acquired syndromes. Nonetheless, determining the exact diagnosis only on the clinical phenotype can be challenging, and a detailed search for associated symptoms is often of crucial importance. As pigmentation
Agnieszka M Topolska-Woś et al.
Nucleic acids research, 48(4), 2173-2188 (2020-01-12)
The XPA protein functions together with the single-stranded DNA (ssDNA) binding protein RPA as the central scaffold to ensure proper positioning of repair factors in multi-protein nucleotide excision repair (NER) machinery. We previously determined the structure of a short motif
Lucia Borszéková Pulzová et al.
International journal of molecular sciences, 21(6) (2020-04-03)
The nucleotide excision repair (NER) pathway is activated in response to a broad spectrum of DNA lesions, including bulky lesions induced by platinum-based chemotherapeutic agents. Expression levels of NER factors and resistance to chemotherapy has been examined with some suggestion
Paul Verbruggen et al.
PLoS computational biology, 10(1), e1003438-e1003438 (2014-02-07)
DNA repair and other chromatin-associated processes are carried out by enzymatic macromolecular complexes that assemble at specific sites on the chromatin fiber. How the rate of these molecular machineries is regulated by their constituent parts is poorly understood. Here we
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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