V3003
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR) antibody produced in mouse
clone 260.4, KDR-2, ascites fluid
Synonyme(s) :
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 antibody produced in mouse, Anti-KDR, Anti-VEGFR-2
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About This Item
Code UNSPSC :
51111800
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
260.4, KDR-2, monoclonal
Contient
15 mM sodium azide
Espèces réactives
human
Technique(s)
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
western blot: 1:500 using insect cell culture supernatant expressing human extracellular recombinant VEGFR or VEGF Receptor-2 (KDR, FLK-1)/FC Chimera human, recombinant
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... KDR(3791)
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Description générale
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (mouse IgG1 isotype) is derived from the KDR-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Vascular endothelial growth factor receptor 2 (VEGFR2) is a type V receptor tyrosine kinase, also known as kinase insert domain receptor (KDR) or fetal liver kinase 1 (Flk-1). It a glycosylated protein with an immunoglobulin (Ig)-like domains, a transmembrane domain and a tyrosine kinase domain. The KDR gene is mapped to human chromosome 4q12 and is widely expressed in all vessel-derived endothelial cells and hematopoietic stem cells.
Spécificité
Reacts specifically with human VEGF receptor-2. This clone has also been termed 260.4. It does not recognize Flt1 (VEGFR-1), Flt4 (VEGFR-3), or platelet-derived growth factor receptor (PDGF-R).
Immunogène
purified recombinant human extracellular VEGF receptor-2 (KDR).
Application
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR) antibody produced in mouse has been used in:
- immunofluorescence
- immunohistochemistry
- immunoblotting
- enzyme-linked immunosorbent assay(ELISA)
Actions biochimiques/physiologiques
Vascular Endothelial Growth Factor Receptor-2 (VEGF R2) have high affinity for vascular endothelial growth factor-A (VEGF-A), hence facilitates most of the endothelial growth and survival signals from VEGF-A. It also plays a pivotal role in vascular development, vascular permeability, embryonic hematopoiesis and regulation of angiogenesis.
Vascular endothelial growth factor receptor 2 (VEGFR2) or kinase insert domain receptor (KDR) is a VEGF receptor, which stimulates its mitogenic activity. It is crucial for vascular endothelial cell development. VEGFR2 expression is elevated in invasive breast cancer, hemangiomas and angiosarcomas. KDR expressing cells show striking changes in cell morphology, actin reorganization and membrane ruffling, chemotaxis, and mitogenicity in response to VEGF. Antibodies that react specifically with the VEGF receptor are useful for the study of the specific differential tissue expression and intracellular localization of the receptor in normal and neoplastic tissue.
Description de la cible
The KDR receptor (VEGFRa), defining marker of hematopoietic stem cells (HSCs), distinguishes pluripotent HSCs from lineage-committed progenitor HSCs.
Forme physique
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
S Ogawa et al.
The Journal of biological chemistry, 273(47), 31273-31282 (1998-11-13)
Vascular endothelial growth factor (VEGF) mediates endothelial cell proliferation, angiogenesis, and vascular permeability via the endothelial cell receptors, KDR/Flk-1 and Flt-1. Recently, a gene encoding a polypeptide with about 25% amino acid identity to mammalian VEGF was identified in the
Masabumi Shibuya
Cancer science, 94(9), 751-756 (2003-09-12)
Vascular endothelial growth factor receptor-2 (VEGFR-2/KDR/Flk-1) is a high-affinity receptor for vascular endothelial growth factor-A (VEGF-A), and mediates most of the endothelial growth and survival signals from VEGF-A. VEGFR-2 has a typical tyrosine kinase receptor structure with seven immunoglobulin (Ig)-like
J M Ou et al.
European journal of histochemistry : EJH, 58(1), 2263-2263 (2014-04-08)
Angiogenesis is a process of development and growth of new capillary blood vessels from pre-existing vessels. Angiogenic growth factors play important roles in the development and maintenance of some malignancies, of which vascular endothelial growth factor (VEGF)/VEGFR2 interactions are involved
I Satriotomo et al.
Neuroscience, 322, 479-488 (2016-03-06)
Repetitive acute intermittent hypoxia (rAIH) increases growth/trophic factor expression in respiratory motor neurons, thereby eliciting spinal respiratory motor plasticity and/or neuroprotection. Here we demonstrate that rAIH effects are not unique to respiratory motor neurons, but are also expressed in non-respiratory
Nikola Holtkamp et al.
Neuro-oncology, 9(3), 291-297 (2007-05-17)
A subset of glioblastomas (GBMs) carry gene amplifications on chromosomal segment 4q12. To characterize this amplicon in detail, we analyzed a set of 100 samples consisting of 65 GBMs, 10 WHO grade III astrocytomas, 12 oligodendrogliomas, and 13 glioma cell
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