U6875

Uridine 5′-triphosphate tris salt

Type VI, ≥90%

• Synonyme(s) : UTP
• Formule empirique (notation de Hill): C₉H₁₅N₂O₁₅P₃
• Numéro CAS: 108321-53-5
• Poids moléculaire : 484.14
• Numéro MDL: MFCD00058360
• Code UNSPSC : 12352200
• ID de substance PubChem : 329827645
• Nomenclature NACRES : NA.77

Propriétés

• Type: Type VI
• Pureté: ≥90%
• Solubilité: H₂O: soluble 50 mg/mL, clear, colorless
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Chaîne SMILES: NC(CO)(CO)CO.O[C@H]1[C@@H](O)[C@@H](O[C@@H]1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O
• InChI: 1S/C9H15N2O15P3.C4H11NO3/c12-5-1-2-11(9(15)10-5)8-7(14)6(13)4(24-8)3-23-28(19,20)26-29(21,22)25-27(16,17)18;5-4(1-6,2-7)3-8/h1-2,4,6-8,13-14H,3H2,(H,19,20)(H,21,22)(H,10,12,15)(H2,16,17,18);6-8H,1-3,5H2/t4-,6-,7-,8-;/m1./s1
• Clé InChI: NHMNWWVJZZPKSL-IAIGYFSYSA-N

Description

Application

UTP has been used to study intracellular Ca²⁺ concentration in alveolar type II (AT2) cells. UTP has also been used as a phosphate donor for in vitro kinase assays using deoxycytidine kinase (DCK) or whole cell lysates.

Uridine 5′-triphosphate tris salt has been used:

• to investigate its activation potential on the purinergic P2Y2 Receptor in THP1 monocytes
• as a component of non-radioactive nucleoside triphosphate solution used in in vitro RNA polymerase assay
• as a P2Y2 agonist in human embryonic kidney cells (HEK293T) in dynamic mass redistribution (DMR) assays

Actions biochimiques/physiologiques

Uridine 5′-triphosphate (UTP) is synthesized from uridine 5′-diphosphate (UDP) in the presence of the enzyme nucleoside diphosphate kinase (NDPK). Plant cells have high uridine as UTP. UTP on amination results in the generation of CTP and is catalysed by enzyme CTP synthetase. UTP acts on the purinergic (P2Y2) receptor and mediates smooth muscle cells (SMCs) mitogenic activation. It also modulates osteopontin expression in SMCs. UTP is a sputum inducer and a promising candidate for assessing the inflammation in respiratory airway.

P2Y receptor agonist.

Notes préparatoires

Uridine 5′-triphosphate (UTP) tris salt is soluble in water at 50 mg/ml and yields a clear, colorless solution.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges