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TP0100

Sigma-Aldrich

Total Protein Kit, Micro

Synonyme(s) :

Bradford

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Description

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

Paramètres

18-26 °C temperature
50 μL sample volume

Température de stockage

2-8°C

Description générale

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Actions biochimiques/physiologiques

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Adéquation

Suitable for protein determination for samples of up to 0.5 mg/ml.

Principe

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Méthode d'analyse

Colorimetric, Endpoint

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • Protein Standard 5 mL

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

Organes cibles

Eyes,Central nervous system

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

132.8 °F

Point d'éclair (°C)

56 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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