T9577

Anti-p8/TTD-A (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : Anti-C6orf175 TFB5, Anti-Chromosome 6 open reading frame 175, Anti-GTF2H5, Anti-General transcription factor IIH, polypeptide 5, Anti-TFIIH basal transcription factor complex, TTDA subunit, Anti-TGF2H5, Anti-TTDA transcription factor IIH, 8-kd subunit
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~8 kDa
• Espèces réactives: mouse, rat, human
• Conditionnement: antibody small pack of 25 μL
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): indirect immunofluorescence: 1:200-1:400 using paraformaldehyde-fixed HEK-293T cells, over-expressing human p8/TTD-A; western blot: 1:250-1:500 using lysates of CHO or HEK-293T over-expressing human p8/TTD-A
• Numéro d'accès UniProt: Q6ZYL4
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GTF2H5(404672) ; mouse ... Gtf2h5(66467) ; rat ... Gtf2h5(502227)

Description

Description générale

8-kDa trichothiodystrophy group A (p8/TTD-A) protein is the tenth subunit of general transcription factor IIH (TGFIIH). It is present as both, bound to transcription factor IIH (TFIIH) as well as a free fraction that shuffles between the cytoplasm and nucleus. The p8/TTD-A gene is located on the human chromosome at 6q25.3.

Spécificité

Anti-p8/TTD-A (N-terminal) specifically recognizes human, rat, and mouse p8/TTD-A.

Application

Anti-p8/TTD-A (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

The 8-kDa trichothiodystrophy group A (p8/TTD-A) protein is required for the stability of the transcription factor IIH (TFIIH) complex. The cells from patients with p8/TTD-A have reduced levels of TFIIH while overexpressing p8/TTD-A restored the cellular level TFIIH complex. Defects in the p8/TTD-A gene are responsible for the third group of the sun-sensitive form of trichothiodystrophy (TTD), a rare autosomal recessive disorder.

Description de la cible

p8/TTD-A (N-terminal) encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)