T8690

Thioredoxin human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

• Numéro CAS: 52500-60-4
• Numéro MDL: MFCD00132423
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli (N-terminal histidine tagged)
• Essai: ≥90% (SDS-PAGE)
• Forme: essentially salt-free, lyophilized powder
• Activité spécifique: ≥5 U/mg
• Poids mol.: 14 kDa
• Technique(s): activity assay: suitable
• Solubilité: water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan
• Adéquation: suitable for molecular biology
• Numéro d'accès UniProt: P10599
• Application(s): cell analysis
• Température de stockage: −20°C
• Informations sur le gène: human ... TXN(7295)

Description

Description générale

Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank^® Accession Number X77584) and the thioredoxin from human placenta (GenBank^® Accession Number AF085844).

Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.[1]Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms.[2]

Application

Thioredoxin human can be used for in vitro thioredoxin assay.[3] The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity.[4]

Thioredoxin human has been used:

• in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay[5]
• to study its role in transcription regulation, RNA processing and nuclear pore function in human cells[6]
• in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice[7]

Actions biochimiques/physiologiques

Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.[8]The product also stimulates growth in normal as well as leukemic B cell,[9] modifies the transcriptional activity of AP-1[10]and inhibits the apoptosis signal-regulating kinase 1 (ASK1).[11]Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent.[2]

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma.[1] It contributes to protein reduction, tissue development and redox regulation.[5]

Définition de l'unité

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Informations légales

GenBank is a registered trademark of United States Department of Health and Human Services

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)