T2815

Transforming Growth Factor-β2 human

TGF-β2, recombinant, expressed in NSO cells, powder, suitable for cell culture

• Synonyme(s) : hTGF-β2, TGF-β2
• Numéro MDL: MFCD00163957
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in NSO cells
• Pureté: ≥97% (electrophoresis)
• Forme: powder
• Puissance: 0.04 ng/mL ED₅₀
• Qualité: endotoxin tested
• Poids mol.: 25 kDa
• Conditionnement: pkg of 10 μg; pkg of 2 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg
• Adéquation: suitable for
• Numéro d'accès UniProt: P61812
• Température de stockage: −20°C
• Informations sur le gène: human ... TGFB2(7042) , TGFBR2(7048)

Description

Description générale

The transforming growth factor-β2 (TGFB2) gene is mapped to human chromosome 1q41. The encoded protein is localized to extracellular matrix. TGF-β2 is a member of the TGF-β superfamily. TGF-β exists in three isoforms (TGF-β1, TGF-β2 and TGF-β3) and are known to share similar structure and function.

Application

Transforming Growth Factor-β2 human has been used in chondrogenic, adipogenic and osteogenic differentiation of mesenchymal stem cells. It has also been used to induce extracellular matrix accumulation in primary human trabecular meshwork cells and further to study the action of tetramethylpyrazine on the same.

Actions biochimiques/physiologiques

TGF-β2 is known to stimulate cell growth, differentiation, migration and survival. TGF-β2 acts as a key regulator for immune homeostasis by controlling lymphocyte proliferation, embryogenesis, hematopoiesis and apoptosis. TGF-β is also associated with inflammation and wound healing and is considered as a strong growth inhibitor for keratinocyte cells. TGF-β is associated with the process of tumor development, progression and metastasis. TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions.

Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.1 mg bovine serum albumin

Remarque sur l'analyse

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)