SRP6556

Thrombin Active (High Activity) from bovine plasma

≥98% (SDS-PAGE), recombinant, lyophilized

• Synonyme(s) : Activated Factor IIa
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Nom du produit: Thrombin Active (High Activity) from bovine plasma, ≥98% (SDS-PAGE)
• Source biologique: bovine plasma
• Essai: ≥98% (SDS-PAGE)
• Forme: lyophilized
• Puissance: >1500 units/mg
• Poids mol.: 37 kDa
• Conditionnement: pkg of 10,000 units; pkg of 100,000 units; pkg of 1000 units
• Numéro d'accès UniProt: P00735
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: bovine ... F2(280685)

Description

Description générale

Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions.[1]

Actions biochimiques/physiologiques

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands.[2] Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII.[1] Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet.[3] As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs.[4] After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. Thrombin enzyme (Activated Factor IIa) also participates in inflammation and has mitogenic role on vascular cells.[5] It is also involved in the activation of protein C which is needed for the inactivation of many procoagulant enzymes.[6]

Forme physique

Sterile filtered and lyophilized with Mannitol and Sodium Chloride.

Reconstitution

Reconstitute in sterile water (100 U/mL) with 0.9% NaCl. It forms a clear solution.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable