SRP6439

CD62E/E-Selectin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonyme(s) : E-Selectin, ELAM1, ESEL, LECAM2, RP1-117P20.2, SELE
• Code UNSPSC : 12352200

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Essai: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: calculated mol wt 60 kDa; observed mol wt 110 kDa by SDS-PAGE (DTT-reduced. Trp22 is the predicted N-terminal.)
• Conditionnement: pkg of 100 μg; pkg of 20 μg
• Numéro d'accès UniProt: P16581
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... SELE(6401)

Description

Description générale

Selectins are a family of calcium-dependent type 1 transmembrane proteins. Endothelial (E)-selectin is a heavily glycosylated transmembrane protein expressed by activated endothelial cells in microvascular linings. E-selectin consists of an N-terminal type 1 lectin domain, an EGF-like domain, 6 sushi (CCP/SCR) domains, a transmembrane sequence, and a short cytoplasmic domain. Recombinant human E-selectin is a 58.6kDa protein containing 535 amino acid residues, corresponding to the extracellular portion of the full length protein. Due to glycosylation, E-selectin migrates at an apparent molecular weight of approximately 65-85kDa by SDS-PAGE analysis under reducing conditions. The gene encoding this protein is localized on human chromosome 1q24.2.[1][2]

Actions biochimiques/physiologiques

E-selectin, along with P-selectin and L-selectin, initiate recruitment of circulating leukocytes from blood to sites of inflammation in the vascular lining through interaction with specific cell surface associated carbohydrate determinants. It is a pro-angiogenic factor. This protein has also been implicated in the pathogenesis of stroke and atherosclerosis.[3][4]

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable