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Principaux documents

SRP6312

Sigma-Aldrich

Alpha 1 Antitrypsin from human plasma

HBsAg, HCV, HBc free, ≥95% (SDS-PAGE)

Synonyme(s) :

AAT, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, PI, Serpin A1

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Essai

≥95% (SDS-PAGE)

Forme

lyophilized

Puissance

≥5.0 I.U. per mg

Poids mol.

52 kDa

Conditionnement

pkg of 1 mg

Numéro d'accès UniProt

P01009

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... Serpin A1(5265)

Description générale

Our Alpha 1 Antitrypsin is a human plasma protein.It is shown to be HBsAg, HCV, HBc free and also negative for anti HIV1 and HIV2 according to FDA approved tests.
The gene AAT (α 1 antitrypsin) is mapped to human chromosome 14q32.1.[1] AAT is an acute-phase plasma protein.[2] It is a water soluble glycoprotein. AAT is mainly secreted by hepatocytes, macrophages, and some cancer cells.[3] AAT is also referred to as Serpin A1.

Application

AAT (α 1 antitrypsin) from human plasma has been used as a standard to check the presence of AAT in benign and malignant lymphoreticular cells and tissues.[4]

Actions biochimiques/physiologiques

AAT (α 1 antitrypsin) functions as a serine protease inhibitor with targets like elastase, plasmin, collagenase, thrombin, leucocyte proteases, trypsin, chymotrypsin and plasminogen activator.[3][5] Clinically, its deficiency is associated with two major diseases: pulmonary emphysema and early onset/juvenile hepatic cirrhosis.[3][6] It is elevated in inflammatory conditions, malignancies, liver disease and pregnancy and also after surgical trauma and use of oral contraceptives.[7][8] The simultaneous quantitative determination of AAT and ceruloplasmin permits differential diagnosis of liver afflictions. Rise in AAT occurs as an acute phase response to tissue necrosis and inflammation.[9] It might work as a biomarker for cutaneous squamous cell carcinoma, hepatitis B, insulinomas, NSCLC (non-small cell lung cancer), papillary thyroid carcinoma, lung cancer and breast carcinoma.[10]

Forme physique

Lyophilized from 30 mM Na Phosphate, pH 6.5 with 300 mM NaCl.

Reconstitution

In water or aqueous buffer

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
8K18L72940
7M20L72940
8J19L72940
7K21L72940
7A28L72940

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Alpha 1 antitrypsin deficiency: the clinical and physiological features of pulmonary emphysema in subjects homozygous for Pi type Z. A survey by the British Thoracic Association.
Tobin MJ
British Journal of Diseases of the Chest, 77, 14-27 (1983)
Impaired hepcidin expression in alpha-1-antitrypsin deficiency associated with iron overload and progressive liver disease.
Schaefer B
Human Molecular Genetics, 24, 6254-6263 (2015)
SERPINA2 is a novel gene with a divergent function from SERPINA1.
Marques PI
PLoS ONE, 8, e66889-e66889 (2013)
Alpha-1-antitrypsin in human macrophages.
Isaacson P
Journal of Clinical Pathology, 34, 982-990 (1981)
Influence of oral contraceptives of differing dosages on alpha-1-antitrypsin, gamma-glutamyltransferase and alkaline phosphatase.
Herbeth B
Clinica Chimica Acta; International Journal of Clinical Chemistry, 112, 293-299 (1981)
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Questions

1–2 sur 2 questions  

  1. How is the amount of Alpha 2-Antiplasmin in the vial supplied (lyophilized) determined? Specifically, which assay is used to determine the amount of Alpha 2-Antiplasmin (100 ug)?

    1 réponse

    1. The protein concentration is measured by its absorbance at A280, utilizing an extinction coefficient of 0.703 for a 0.1% solution at a 1 cm path length.

      Utile ?

  2. What is the correct molecular weight for alpha 2 antiplasmin?

    1 réponse

    1. The predicted molecular weight of human alpha-2 antiplasmin, based on its amino acid sequence, is approximately 50,500. However, when 11-14% carbohydrate content is considered, the molecular weight increases to a maximum of about 58,000. Despite this, physical measurements of the molecular weight have varied from 63,000 to 70,500.

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