SRP6169

IL-1β human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonyme(s) : Catabolin, Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Lymphocyte-activating factor (LAF), Mononuclear cell factor (MCF)
• Numéro CAS: 97599-21-8
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: (18 and 25 kDa, monomer, glycosylated)
• Conditionnement: pkg of 10 μg
• Technique(s): cell culture | mammalian: suitable
• Numéro d'accès NCBI: P01584
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... IL1B(3553)

Description

Description générale

Interleukin-1β (IL1B) is produced by activated macrophages. It is synthesized as pro-IL-1β which is changed to active form IL-1β with the help of pro-inflammatory protease caspase-1. IL-1β belongs to the IL-1 gene family. It is a proinflammatory cytokine. The gene is mapped to human chromosome 2q14. In comparison with the Escherichia coli expressed protein, IL-1β is 50% more potent in promoting human CD4+ T cell differentiation into Th17 (T helper) cells. This cytokine is produced in a serum free, chemically defined media.

Application

• IL-1β (IL1B) has been used to check the expression of NGAL (neutrophil gelatinase-associated lipocalin) in epithelial cells after stimulation with proinflammatory cytokines.
• It has been used to check anti-inflammatory activities of N-Acetylglucosamine in human chondrocytes.
• It has been used to create inflammatory endothelium in human coronary artery endothelial cells.

Actions biochimiques/physiologiques

IL-1β (IL1B) stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation and fibroblast growth factor activity. IL-1β proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1β is present in chronic inflamed tissues and also in tissues going for fibrogenesis. It brings neutrophil and macrophage to the site of infection. Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura. It is also associated with destruction of β-cell in type 1 diabetes.

Forme physique

Lyophilized from a PBS solution.

Notes préparatoires

Centrifuge the vial prior to opening.

Reconstitution

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable