This product is tested under both unreduced and reduced conditions. Under unreduced conditions, the SDS-PAGE gel shows a band at 26 kDa, while under reduced conditions, the only band is around 13 kDa molecular weight.
SRP3003
Activin A human
recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
Synonyme(s) :
Activin beta-A, FRP, Inhibin beta-1
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
10 μg | Vérifier la disponibilité des articles du panier | 358,00 € |
A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352202
358,00 €
Vérifier la disponibilité des articles du panier
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human
recombinant
expressed in E. coli
assay
≥97% (HPLC), ≥97% (SDS-PAGE)
form
lyophilized
potency
≤2.0 ng/mL ED50
mol wt
26.0 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... INHBA(3624)
mouse ... Inhba(16323)
rat ... Inhba(29200)
General description
Activins (also referred to as inhibins) are generally dimers of three subunits: inhibin βA, inhibin βB, and inhibin α. Recombinant human Activin A (inhibin βA) is a 26kDa disulfide-linked homodimer of two bA chains, each containing 116 amino acid residues. The sequence of mature Activin A is completely homologous between human, mouse, and rat species.
Application
Activin A has been used in monolayer induction for endoderm (hepatocyte) differentiation.
Activin A human has been used to study rRNA gene transcription during directed differentiation of human embryonic stem cells.
Biochem/physiol Actions
Activin A (inhibin βA) is a TGF (transforming growth factor)-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively. Activin A plays a central role in reproduction by regulating the levels of follicle stimulating hormone (FSH). It is involved in hematopoiesis, embryogenesis and osteogenesis. It exhibits both tumor suppressing and promoting effects. In underweight and overweight humans, high levels of Activin A in serum might be associated with the pathogenesis of asthma. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
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Cet article | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture | description TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture | description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture | description ≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, lyophilized powder, suitable for cell culture |
| assay ≥97% (HPLC), ≥97% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥98% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source human | biological source human | biological source human | biological source human |
| recombinant expressed in E. coli | recombinant expressed in CHO cells | recombinant expressed in E. coli | recombinant expressed in baculovirus infected insect cells |
| form lyophilized | form powder | form lyophilized | form lyophilized powder |
| mol wt 26.0 kDa | mol wt dimer (12.8/25.6 kDa (112/224 aa)) | mol wt (Noncovalent Homodimer, 13.6 kDa (121 amino acids)) | mol wt dimer 26 kDa (containing 116 amino acid residues βA subunits) |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How does this protein appear on a non-reducing SDS-PAGE? Should it be present in its monomeric or its dimeric form? Or does it occur in variable ratios of the two? Does the amount of protein applied, influence its banding pattern?
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