SRP3003

Activin A human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

• Synonyme(s) : Activin beta-A, FRP, Inhibin beta-1
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Essai: ≥97% (HPLC); ≥97% (SDS-PAGE)
• Forme: lyophilized
• Puissance: ≤2.0 ng/mL ED₅₀
• Poids mol.: 26.0 kDa
• Conditionnement: pkg of 10 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg endotoxin, tested
• Numéro d'accès UniProt: P08476
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... INHBA(3624) ; mouse ... Inhba(16323) ; rat ... Inhba(29200)

Description

Description générale

Activins (also referred to as inhibins) are generally dimers of three subunits: inhibin βA, inhibin βB, and inhibin α.[1] Recombinant human Activin A (inhibin βA) is a 26kDa disulfide-linked homodimer of two bA chains, each containing 116 amino acid residues. The sequence of mature Activin A is completely homologous between human, mouse, and rat species.

Application

Activin A has been used in monolayer induction for endoderm (hepatocyte) differentiation.[2]

Activin A human has been used to study rRNA gene transcription during directed differentiation of human embryonic stem cells.[3]

Actions biochimiques/physiologiques

Activin A (inhibin βA) is a TGF (transforming growth factor)-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival.[4] Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively.[1][5] Activin A plays a central role in reproduction by regulating the levels of follicle stimulating hormone (FSH). It is involved in hematopoiesis, embryogenesis and osteogenesis. It exhibits both tumor suppressing and promoting effects.[6] In underweight and overweight humans, high levels of Activin A in serum might be associated with the pathogenesis of asthma.[7] The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.[8]

Séquence

GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Forme physique

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable