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SRP0461

Sigma-Aldrich

BRD4 (49-170) His tag human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonyme(s) :

CAP, HUNK1, HUNKI, MCAP, bromodomain containing 4

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥90% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

15.5 kDa

Conditionnement

pkg of 100 μg

Technique(s)

cell based assay: suitable

Solubilité

water: soluble

Numéro d'accès NCBI

NM_058243

Numéro d'accès UniProt

O60885

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... BRD4(23476)

Description générale

Research area: Cancer

BRD4 (bromodomain-containing protein 4) belongs to the BET (bromodomain and extra-terminal) family. The protein has two bromodomains (BD1 and BD2) connected via a long interdomain linker.Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP (GenBank Accession No. NM_058243) amino-acids 49-170 with N-terminal His-tag, MW = 15.5kDa, expressed in an Escherichia coli expression system.

Actions biochimiques/physiologiques

BRD4 (bromodomain-containing protein 4) works as a reader of epigenetic marks and is involved in chromatin remodeling and transcriptional regulation. Therefore, it is a target for oncology, inflammation and cardiovascular disease. This gene is upregulated in hepatocellular carcinoma and is associated with poor prognosis. BRD4 is also associated with melanoma, hepatocellular carcinoma, multiple myeloma, Burkitt′s lymphoma, acute myeloid leukemia and breast cancer. BRD4 connects cell cycle and transcription, works as a scaffold for transcription factors and bookmarks active genes during mitosis. It interacts with acetylated histone-3 and histone-4 tails and helps in maintaining chromatin architecture. Also, it regulates transcription elongation via phosphorylation of RNA polymerase II.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Segmental Isotope Labelling of an Individual Bromodomain of a Tandem Domain BRD4 Using Sortase A.
Williams FP, et al.
PLoS ONE, 11, e0154607-e0154607 (2016)
Philipp Voigt et al.
Genome biology, 12(11), 133-133 (2011-12-01)
The chromatin adapter BRD4 may be crucial for transmitting epigenetic information by acting as a histone acetylation-dependent gene bookmark and accelerating post-mitotic transcriptional reactivation.
BRD4 promotes tumor growth and epithelial-mesenchymal transition in hepatocellular carcinoma.
Zhang P, et al.
International Journal of Immunopathology and Pharmacology, 28, 36-36 (2015)
High level of BRD4 promotes non-small cell lung cancer progression.
Liao YF, et al.
Oncotarget, 7, 9491-9491 (2016)
R M Rodriguez et al.
Journal of molecular medicine (Berlin, Germany), 90(5), 587-595 (2011-11-29)
The bromodomain protein BRD4 is involved in cell proliferation and cell cycle progression, primarily through its role in acetylated chromatin-dependent regulation of transcription at targeted loci. Here, we show that BRD4 is frequently downregulated by aberrant promoter hypermethylation in human

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