SRP0135

G9a human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonyme(s) : EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Essai: ≥70% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 74.6 kDa
• Conditionnement: pkg of 50 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_006709
• Numéro d'accès UniProt: Q96KQ7
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... EHMT2(10919)

Description

Description générale

Human G9a (euchromatic histone-lysine N-methyltransferase 2) with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. G9a is a member of SET (Su(var)3-9, enhancer-of-zeste and trithorax) domain containing, Su(var)3-9 family of proteins. This domain is evolutionary conserved.[1]

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

The encoded protein G9a (euchromatic histone-lysine N-methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3.[2] Overexpression of G9a is observed in cancer. It is known to participate in cancer cell migration, invasion and metastasis.[3] G9a is an epigenetic regulator and can inhibit the action of tumor suppressor genes, thereby, contributes to carcinogenesis. Thus, G9a is considered to be an oncogenic and metastatic factor.[4] It also affects DNA damage repair mechanism, which might make tumor cells resistant to radiation and chemotherapy.[5] G9a serves as a target for cancer therapy including breast cancer.[4]

Définition de l'unité

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable