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SAB4503685

Sigma-Aldrich

Anti-CEP135 antibody produced in rabbit

affinity isolated antibody

Synonyme(s) :

Centrosomal protein 4, Centrosomal protein of 135 kDa, Cep135

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 133 kDa

Espèces réactives

mouse, human

Concentration

~1 mg/mL

Technique(s)

ELISA: 1:40000
western blot: 1:500-1:1000

Numéro d'accès NCBI

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CP135(9662)

Description générale

Anti-CEP135 Antibody detects endogenous levels of total CEP135 protein.
Cep135 (centrosomal protein 135) is a cartwheel protein. It has a coiled-coil domain and conserved regions near the N-terminus and the C-terminus, respectively. It is mapped to human chromosome 4q12.

Immunogène

The antiserum was produced against synthesized peptide derived from human CEP135.

Immunogen Range: 1081-1130

Application

Anti-CEP135, C-Terminal antibody has been used in immunofluorescence staining.

Actions biochimiques/physiologiques

Mutation in Cep135 (centrosomal protein 135) result in primary microcephaly. It induces centriole assembly and stability. CEP135 helps to maintain the structure and organization of microtubules. This protein plays a major role in centriole biogenesis.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

A truncating mutation of CEP135 causes primary microcephaly and disturbed centrosomal function
Hussain MS, et al.
American Journal of Human Genetics, 90(5), 871-878 (2012)
A Short CEP135 Splice Isoform Controls Centriole Duplication
Dahl KD, et al.
Current Biology, 25(19), 2591-2596 (2015)
A novel splice site mutation in CEP135 is associated with primary microcephaly in a Pakistani family
Farooq M, et al.
Journal of Human Genetics, 61(3), 271-273 (2016)
Control of apico-basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7
Noordstra I, et al.
Journal of Cell Science, 129(22), 4278-4288 (2016)
Ivar Noordstra et al.
Journal of cell science, 129(22), 4278-4288 (2016-11-02)
The microtubule cytoskeleton regulates cell polarity by spatially organizing membrane trafficking and signaling processes. In epithelial cells, microtubules form parallel arrays aligned along the apico-basal axis, and recent work has demonstrated that the members of CAMSAP/Patronin family control apical tethering

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