SAB4200408

Anti-Dystrobrevin-α antibody produced in rabbit

IgG fraction of antiserum

• Synonyme(s) : AntiDTNA
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~55 kDa
• Espèces réactives: human
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): indirect immunofluorescence: 1:10,000-1:20,000 using HEK-293T cells overexpressing human dystrobrevin-a variant 6; western blot: 1:5,000-1:10,000 using lysates of HEK-293T cells overexpressing human dystrobrevin-a variant 6
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DTNA(1837)

Description

Description générale

Dystrobrevin-α (α-DB or DTNA) belongs to the dystrobrevin (DB) subfamily of the dystrophin-related-protein family.α-DB is a component of the dystrophin-glycoprotein complex (DGC) that is located at the muscle sarcolemma and forms a transmembrane link between the cytoskeleton and the basal lamina. The DB family includes two isoforms α- and β-DB encoded by the different genes. α-DB is also abundantly expressed in developing neurons and in the adult central nervous system (CNS). The DTNA gene is located on the human chromosome at 18q12.1.

Spécificité

Anti-Dystrobrevin-α specifically recognizes human dystrobrevin-α.

Immunogène

synthetic peptide corresponding to a sequence at the C-terminal region of human dystrobrevin-a (DTNA), conjugated to KLH

Application

Anti-Dystrobrevin-α antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

Dystrobrevin- α (α -DB) is involved in the formation and stability of synapses and the clustering of nicotinic acetylcholine receptors. Mutations in the DTNA gene are associated with congenital heart defects. Dystrobrevin is markedly reduced or absent in the sarcolemma of patients with Duchenne muscular dystrophy (DMD).

Forme physique

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable