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SAB4200393

Sigma-Aldrich

Anti-BRSK1 (C-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-BR SERINE/THREONINE KINASE 1, Anti-SAD-B

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~90 kDa

Espèces réactives

human, mouse

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Concentration

~1.5 mg/mL

Technique(s)

immunoprecipitation (IP): 10-20 μg using HEK-293T cell lysates overexpressing mouse BRSK1.
indirect immunofluorescence: 0.2-0.4 μg/mL using HEK-293T cells overexpressing mouse BRSK1.
western blot: 1-2 μg/mL using mouse forebrain (S1 fraction).

Numéro d'accès UniProt

Q8TDC3

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... BRSK1(84446)
mouse ... Brsk1(381979)

Description générale

BRSK1 is a serine/threonine kinase that facilitates the G(2)/M cell cycle arrest upon UV- or methyl methane sulfonate-induced DNA damage. This kinase also modulates the polarity of neurons and duplication of centrosomes . Anti-BRSK1 (C-terminal) antibody is specific for mouse and human BRSK1. In immunoblotting, detection of the BRSK1 band is specifically inhibited by the BRSK1 immunizing peptide.

Immunogène

synthetic peptide corresponding to a sequence at the C-terminal of human BRSK1, conjugated to KLH. The corresponding sequence is identical in mouse BRSK1.

Application

Anti-BRSK1 (C-terminal) antibody is suitable for use in western blot (1-2 μg/mL using S1 fraction extracts of mouse forebrain). The product can also be used for immunoprecipitation (10-20 μg) and indirect immunofluorescence (0.2-0.4 μg/mL) using HEK-293T cells overexpressing mouse BRSK1.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
121M4772

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

María Alvarado-Kristensson et al.
Nature cell biology, 11(9), 1081-1092 (2009-08-04)
Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a
Rui Lu et al.
The Journal of biological chemistry, 279(30), 31164-31170 (2004-05-20)
Checkpoint activation by DNA damage during G(2) prevents activation of cyclin B/Cdc2 complexes, and as a consequence, mitotic entry is blocked. Although initiation and maintenance of G(2) arrest are known to be regulated by at least two distinct signaling pathways
Myriam Müller et al.
Journal of cell science, 123(Pt 2), 286-294 (2009-12-23)
Wee1 is well characterized as a cell-cycle checkpoint kinase that regulates the entry into mitosis in dividing cells. Here we identify a novel function of Wee1 in postmitotic neurons during the establishment of distinct axonal and dendritic compartments, which is

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