SAB4200379

Anti-JMY antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonyme(s) : Anti-Junction mediating and regulatory protein, Anti-p53 cofactor
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~120 kDa
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunoprecipitation (IP): 5-10 μg using lysates of human WiDr cells.; indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.; western blot: 0.5-1.0 μg/mL using whole extracts of human WiDr cells
• Numéro d'accès UniProt: Q8N9B5
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... JMY(133746)

Description

Description générale

Junction mediating and regulatory protein (JMY) is identified as a transcriptional co-activator of p53 and is a multifunctional actin nucleation factor. JMY contains Wiskott-Aldrich syndrome protein (WASp)-homology 2 (WH2) domains in its C-terminus. The JMY gene is mapped to human chromosome location 5q14.1.

Spécificité

Anti-JMY recognizes human JMY.

Immunogène

synthetic peptide corresponding to the C-terminal region of human JMY, conjugated to KLH.

Application

Anti-JMY antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence.

Actions biochimiques/physiologiques

Junction mediating and regulatory protein (JMY) acts as a nucleation promoting factor by activating actin-related proteins-2/3 (ARP2/3), creating branched filament networks, and also by assembling unbranched filaments directly through a Spire-like mechanism. Overexpression of JMY increases motility while its loss slows cell migration. DNA damage causes JMY to accumulate in the nucleus, where it enhances p53-dependent transcription of pro-apoptotic genes. The activities of JMY as a p53 co-factor or as an actin nucleation factor are regulated by its subcellular localization.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable