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SAB4200353

Sigma-Aldrich

Anti-acetyl-Histone H4 [Ac-Lys12] antibody produced in rabbit

IgG fraction of antiserum

Synonyme(s) :

Anti-H4, Anti-H4K12ac, Anti-HIST4H4, Anti-Histone cluster 4

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~12 kDa

Espèces réactives

mouse, rat, human

Technique(s)

immunohistochemistry: 1:100-1:200 using formalin-fixed paraffin-embedded human breast carcinoma
indirect immunofluorescence: 1:250-1:500 using HeLa cells
western blot: 1:500-1:1000 using NIH3T3 cell lysates

Numéro d'accès UniProt

Q16695

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

acetylation (Lys12)

Informations sur le gène

human ... HIST3H3(8290)

Catégories apparentées

Description générale

Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. It is an important component of chromatin structure.

Immunogène

synthetic peptide containing acetylated Lys12 of human histone H4, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H4.

Application

Anti-acetyl-Histone H4 [Ac-Lys12] antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Actions biochimiques/physiologiques

Histone H4 can be reversibly acetylated at lysine residues K5, K8, K12 and K16, and the acetylation occurs initially at K16 and then propagates in an N-terminal direction through K12, K8 and K5. Altered acetylation of histone H4 on Lys12 (H4- K12Ac) is linked to age-dependent memory impairment in mice. Histones are subjected to several covalent modifications, such as phosphorylation, methylation, acetylation and ubiquitination, that affect chromatin structure and regulate chromatin activity. Histone modifications plays an important role in cancer and disease.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Altered histone acetylation is associated with age-dependent memory impairment in mice
Peleg S, et al.
Science, 328(5979), 753-756 (2010)
Robert Schneider et al.
Trends in biochemical sciences, 27(8), 396-402 (2002-08-02)
Enzymes that covalently modify histones control many cellular processes by affecting gene expression. A new class of these enzymes is the histone lysine methyltransferase family, whose catalytic activity lies within a conserved domain, the SET domain. This article surveys the
The language of covalent histone modifications
Strahl BD and Allis CD
Nature, 403(6765), 41-41 (2000)

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